Cold adaptation of xylose isomerase fromThermus thermophilusthrough random PCR mutagenesis
نویسندگان
چکیده
منابع مشابه
Random mutagenesis by whole-plasmid PCR amplification.
Random mutagenesis has become a powerful means of studying the effects of a large number of permutations of a particular DNA sequence. As a prime example, libraries of randomized cDNA clones, when translated into their corresponding proteins, can be useful in investigating the functional contributions of a mutagenized region to the overall properties of a protein. Existing molecular cloning tec...
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5 Sun-Mi Lee, Taylor Jellison, and Hal S. Alper* 6 7 8 1 Department of Chemical Engineering, The University of Texas at Austin,1 University Station, 9 C0400,Austin, Texas 78712 10 11 2 Clean Energy Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok12 dong, Seongbuk-gu, Seoul 136-791, Korea 13 14 3 Institute for Cellular and Molecular Biology, The University of Texas at Au...
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The gene encoding chitinase ArChiB from the Antarctic Arthrobacter sp. TAD20 has been expressed in Escherichia coli and the recombinant enzyme purified to homogeneity. In an effort to engineer cold-adapted biocatalysts through rational redesign to operate at elevated temperatures, we performed several mutations aiming to increase the rigidity of the molecular edifice of the selected psychrophil...
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Xylose isomerase (XI) catalyzes the isomerization and epimerization of hexoses, pentoses and tetroses. In order to clarify the reasons for the low reaction efficiency of a pentose sugar, L-arabinose, we determined the crystal structure of Streptomyces rubiginosus XI complexed with L-arabinose. The crystal structure revealed that, when compared with D-xylose and D-glucose, L-arabinose binds to t...
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* This work was supported by National Institutes of Health Grants CA-10925, CA-OG927, and RR-05539 from the United States Public Health Service and by an appropriation from the Commonwealth of Pennsylvania. (I), was crystallized from acetone. The enzyme from Bacillus coagulans has also been crystallized from ammonium sulfate solution and from aqueous acetone (3). We describe here preliminary x-...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2002
ISSN: 0014-2956
DOI: 10.1046/j.0014-2956.2002.02631.x